Binding of fluorescent-labeled phosphatidylcholine to rat liver nonspecific lipid transfer protein.
نویسندگان
چکیده
منابع مشابه
Phospholipid binding and transfer by the nonspecific lipid-transfer protein (sterol carrier protein 2). A kinetic model.
The nonspecific lipid-transfer protein (nsL-TP) from bovine liver was studied by measuring the binding and transfer of the fluorescent phospholipid 1-palmitoyl-2-[6-(1-pyrenyl)-hexanoyl]-sn-glycero-3-phosphocholine (PamPryGroPCho). A kinetic model is presented involving three steps: (a) interaction of nsL-TP with a membrane surface; (b) equilibration of PamPyrGroPCho monomers between the membra...
متن کاملBaculovirus-mediated expression of recombinant rat phosphatidylcholine transfer protein.
Phosphatidylcholine transfer protein catalyzes intermembrane transfer of phosphatidylcholines exclusive of all other phospholipid classes. Although postulated to participate in phosphatidylcholine biosynthesis and biliary trafficking in liver, the molecular basis underlying the substrate specificity of phosphatidylcholine transfer protein remains to be elucidated. Having demonstrated the inabil...
متن کاملThe precursor form of the rat liver non-specific lipid-transfer protein, expressed in Escherichia coli, has lipid transfer activity.
The cDNA encoding the precursor form of non-specific lipid-transfer protein (pre-nsL-TP) from rat liver was cloned into the expression vector pET3d. The resulting plasmid was transformed to the Escherichia coli strain BL21(DE3). After induction of the bacteria with isopropyl-beta-D-thiogalactopyranoside (IPTG) pre-nsL-TP was purified from the bacterial lysate by anion exchange chromatography fo...
متن کاملBinding of plasma-derived lipid transfer protein to lipoprotein substrates. The role of binding in the lipid transfer process.
Plasma-derived lipid transfer protein (LTP) facilitates the transfer of cholesteryl ester and triglyceride between all lipoproteins. Kinetic models of the transfer event have suggested that transfer is facilitated through the physical interaction (binding) of LTP with its lipoprotein substrate. Such binding has been observed previously between LTP and high density lipoprotein (HDL), but not bet...
متن کاملImpaired response of biliary lipid secretion to a lithogenic diet in phosphatidylcholine transfer protein-deficient mice.
Phosphatidylcholine transfer protein (PC-TP) is a cytosolic lipid transfer protein that is highly expressed in liver and catalyzes intermembrane transfer of phosphatidylcholines in vitro. To explore a role for PC-TP in the hepatocellular trafficking of biliary phosphatidylcholines, we characterized biliary lipid secretion using Pctp(-/-) and wild-type littermate control mice with C57BL/6J and F...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1987
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)47920-3